2022 Fiscal Year Final Research Report
Analysis of glucose sensing mechanism using live imaging and mathematical modeling
| Project/Area Number |
20K06647
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44010:Cell biology-related
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| Research Institution | Kurume University |
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Principal Investigator |
Sano Hiroko 久留米大学, 付置研究所, 講師 (90506908)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | グルコース感知 / 糖代謝 / Mondo/ChREBP / ショウジョウバエ / マウス |
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| Outline of Final Research Achievements |
To clarify the mechanism by which cells sense changes in glucose uptake, we constructed an experimental system to simultaneously detect intracellular glucose concentration and the activity of the glucose-responsive transcription factor, Mondo. Using a culture system of the fat body, a nutrient-sensing organ of Drosophila larvae, we constructed a live imaging system to monitor intracellular glucose concentration with green fluorescent protein and Mondo activity with red fluorescent protein over time.
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| Free Research Field |
発生遺伝学、代謝内分泌学
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| Academic Significance and Societal Importance of the Research Achievements |
動物の栄養摂取には変動があるため、細胞は常に栄養摂取状態を読み取り、栄養摂取に合わせて代謝を調節することにより代謝恒常性を保っている。ヒトの主要なエネルギー源であるグルコース代謝の恒常性破綻は糖尿病などの疾患につながる可能性があり、グルコース感知の分子機構の解明は疾患発症メカニズムの解明に寄与することが期待される。
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