Elucidation of the molecular mechanism for accurately and efficiently retrograde cargo transport from the Golgi to the ER

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06650
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44010:Cell biology-related
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Kurokawa Kazuo 国立研究開発法人理化学研究所, 光量子工学研究センター, 専任研究員 (40333504)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ゴルジ体 / 4Dライブイメージング / 小胞体 / 積荷受容体

Outline of Final Research Achievements

To elucidate the mechanism of retrograde transport from the Golgi to the ER, I performed simultaneous multicolor 4D live imaging analysis of the cargo receptor Emp24, cis-Golgi, and ERES. Emp24 showed quick anterograde transport to cis-Golgi during the contact between cis-Golgi and the ERES. During this contact, Emp24 was then retrogradely transported to the ERES. It was shown that both anterograde and retrograde transport of the cargo receptor is achieved during the contact of cis-Golgi with the ERES (hug-and-kiss action).

Free Research Field

膜交通（小胞輸送）

Academic Significance and Societal Importance of the Research Achievements

本研究課題では、生きた細胞内の複数の分子の4D動態を捉える多色同時高時空間分解能4Dイメージングと出芽酵母の自由度の高い遺伝学とを組み合わせた研究手法を進めることで、膜交通の新たなモデルを提案する結果を得ることができた。