2022 Fiscal Year Final Research Report
Genetic evidence for the role of cold-induced factors and Flowering Locus C in perennial growth of Arabidopsis haleri gemmifera
| Project/Area Number |
20K06699
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44030:Plant molecular biology and physiology-related
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | vernalization / life history / flowering locus C |
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| Outline of Final Research Achievements |
Flowering Locus C is a central repressor of flowering in the crucifer family. While exceptionally well characterised in winter annual growth habits, its function had not been studied genetically in crucifers with perennial growth habit, which are less amenable genetic systems but they represent the ancestral forms. In this work, we created mutants in the Flowering Locus C ortholgue in Arabidopsis halleri gemmifera, a perennial crucifer from Japan with a unique growth habit. After optimizing several steps of the tissue culture Agrobacterium-mediated transformation in Ahg, we were able to obtain a limited number of primary transgenics expressing siRNA against AhgFLC. Close investigation of the mutant phenotype pointed to several perennial traits that AhgFLC controls, largely in line with expectations. These phenotypes remain to be substantiated in sequent generations. This work contributes to general understanding of the genetic determinants of annual vs perennial growth habits.
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| Free Research Field |
plant development and environmental response
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| Academic Significance and Societal Importance of the Research Achievements |
Vernalization contributed to adaptation to diverse climates and diversification of life histories and was utilised in breeding. Together with work from Arabidopsis thaliana, this work contributes to general understanding of the genetic determinants of annual vs perennial growth habits.
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