2022 Fiscal Year Final Research Report
Functions of membrane steroid receptors and molecular mechanism of oocyte maturation and ovulation
| Project/Area Number |
20K06719
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44040:Morphology and anatomical structure-related
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| Research Institution | Shizuoka University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | ステロイド膜受容体 / 卵成熟 / 排卵 / ゲノム編集 / スクリーニング法 / 天然活性物質 / 遺伝子発現 |
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| Outline of Final Research Achievements |
Some mutant lines of steroid membrane receptor mPR showing developmental abnormalities were obtained. Detailed phenotypic analysis will be conducted in the future. Three candidate ovulation-inducing genes that show clear ovulation-specific expression were selected for further phenotypic analysis. The results were published.
We succeeded in obtaining progesterone-specific reactivity of the steroid membrane receptor mPR molecule by preparing nanoparticles, graphene Q-dot nanoparticles with mPR (mPRα-GQDs). Two fractions of natural hormonal active substances secreted by the seaweed were successfully purified by HPLC and confirmed to be mPR-reactive substances by an assay using mPRα-GQD.
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| Free Research Field |
生殖生物学
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| Academic Significance and Societal Importance of the Research Achievements |
ステロイド膜受容体mPRの個体レベルでの機能証明ができれば、ステロイド膜受容体の研究は世界的に広がることが予想され、ステロイドホルモンのノンゲノミック作用の理解が深まることにより医学、薬学応用へ繋がることが期待される。そこでCRISPR/Cas9法により作出した遺伝子破壊系統の表現型解析により、機能証明を進める必要がある。研究代表者らが開発した新規卵成熟・排卵誘導法を用いて選択した排卵誘導遺伝子群についてもゲノム編集魚を用いて同様の解析を進める。排卵誘導機構が解明されれば排卵障害による不妊の問題への対処法のヒントを与えることも期待される。
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