2022 Fiscal Year Final Research Report
A constructive approach for duplex vision using mathematical and mouse models.
| Project/Area Number |
20K06737
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44050:Animal physiological chemistry, physiology and behavioral biology-related
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 視細胞 / 網膜 / オプシン / 小口病 / ノックインマウス |
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| Outline of Final Research Achievements |
In vertebrate retina, rod and cone photoreceptors are responsible for scotopic and photopic vision, respectively. The light sensitivity and kinetics of the photoreceptors differ depending on their vison. To elucidate the relationship between photoresponse properties and the phototransduction cascades in both photoreceptor cells, we created three mouse models of (1) cyclostome-type rhodopsin (2) rhodopsin-like cone opsin (3) cone-associated Oguchi disease. We found that the conserved residues in rhodopsin group were involved in the light sensitivity of rods, while the residue conserved in cone opsins were involved in the cone response kinetics. Also, we proposed a novel mechanism that causes the cone-associated Oguchi disease.
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| Free Research Field |
動物生理学
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| Academic Significance and Societal Importance of the Research Achievements |
これまで小口病のマウスモデルは、原因遺伝子ノックアウトマウスが用いられてきた。しかし、ノックアウトマウスは桿体の異常に関してはいいモデルであるが、ヒト錐体の光応答の回復に異常がみられる症例について説明できなかった。本研究において作出した錐体関連の小口病マウスモデルから、ヒト錐体の回復異常に発症に関する新規の機序を明らかにすることができた
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