Genetic analysis of BLM-TOP3alpha-RMI1-RMI2 complex

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06760
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 45010:Genetics-related
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: Abe Takuya 東京都立大学, 理学研究科, 助教 (50779999)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 染色体安定性 / DT40 / BLM / RMI1 / RMI2

Outline of Final Research Achievements

In this study, we aimed to understand the mechanism of homologous recombination by clarifying the genetic relationship of the BLM-TOP3alpha-RMI1-RMI2 (BTR) complex, which resolves the DNA strand entanglements produced during homologous recombination process. As a result, we found synthetic lethality between RMI1/RMI2 and revealed that the synthetic lethality was suppressed by deletion of BLM. Considering the results obtained in yeast, it became clear that the regulation of homologous recombination by the BTR complex is a highly conserved mechanism from yeast to higher eukaryotes.

Free Research Field

分子生物学、遺伝学

Academic Significance and Societal Importance of the Research Achievements

BTR複合体を構成するタンパク質をコードする遺伝子の変異はブルーム症候群などの遺伝病の原因となる。これらの遺伝病ではありとあらゆる癌の発生頻度が上昇することから、BTR複合体の機能を理解することは、癌の発生メカニズムの解明、そしてその予防にとっても重要である。本研究で明らかにしたBTR複合体における遺伝学的関係性は今後さらにBTR複合体の機能の詳細を解明するための基盤となるものである。