2021 Fiscal Year Research-status Report
The role of non-allelic gene conversion in the evolution of Transposable Elements
| Project/Area Number |
20K06761
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
FAWCETT JEFFREY 国立研究開発法人理化学研究所, 数理創造プログラム, 上級研究員 (50727394)
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| Co-Investigator(Kenkyū-buntansha) |
印南 秀樹 総合研究大学院大学, 先導科学研究科, 教授 (90444140)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | Transposable Element / gene conversion |
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| Outline of Annual Research Achievements |
Transposable Elements (TEs) are known to have contributed to the rewiring of regulatory networks by providing a large number of binding motifs throughout the genome. We previously argued that non-allelic gene conversion between TEs likely contributes not only to the evolution of TEs, but also to the rewiring of regulatory networks via TEs. Here, we used a previously developed simulation framework to study the sequence evolution of Transposable Elements (TEs). In addition, we have also begun data analysis using B2 SINEs in mouse as a model. This TE family has expanded in rodents and has contributed to CTCF binding sites. We have obtained genome sequences and genomic positions of B2 SINEs in mouse and we are curating these datasets and classifying the B2 SINEs based on their insertion age and involvement in protein binding sites. We will use these datasets to detect non-allelic gene conversion in TEs and discuss the role of non-allelic gene conversion and TEs in the rewiring of regulatory networks. We will use these datasets to detect non-allelic gene conversion in TEs and discuss the role of non-allelic gene conversion and TEs in the rewiring of regulatory networks. I presented these ideas and results of some our theoretical studies at the Annual Meeting of the Molecular Biology Society of Japan.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We have been able to perform simulation of TE evolution and genome sequence analyses using genomic data of mouse
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| Strategy for Future Research Activity |
We will summarize our results of simulation. In addition, we will examine the role of non-allelic gene conversion in the evolution of B2 SINEs, a TE family in rodents, especially focusing on their contribution to CTCF binding
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| Causes of Carryover |
We have not been able to attend scientific meetings and hold on-site meetings due to the COVID-19. We plan to attend scientific meetings and have in-person discussions during the next fiscal year
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