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2022 Fiscal Year Annual Research Report

Investigation of the role of radial glia in regulating LysoPtdGlc required for nervous system development

Research Project

Project/Area Number 20K06884
Research InstitutionKyoto University

Principal Investigator

GUY ADAM・TSUDA  京都大学, 生命科学研究科, 准教授 (30634779)

Project Period (FY) 2020-04-01 – 2023-03-31
KeywordsLipid biology / Developmental biology / Neuroscience / Neurochemistry / Cell biology
Outline of Annual Research Achievements

In this project, I made the discovery of two isomers of the axon guidance cue lysophosphatidylglucoside (LysoPtdGlc) that activate the orphan receptor GPR55 in a potentially novel mechanism. I found that LysoPtdGlc can exist in two biologically active isomeric forms, R-LysoPtdGlc and S-LysoPtdGlc, that are R/S stereoisomers. Whilst both isomers activate GPR55, R-LysoPtdGlc activates Galpha13 downstream of GPR55, whilst S-LysoPtdGlc activates GalphaS, and they induce polar-opposite chemotropic responses of axon growth. I confirmed this using drugs that specifically inhibit components of adenylyl cyclase pathway, and by specifically blocking different Galpha subunit pathways with inhibitory peptides. In summary, I have discovered a potential ligand bias or functional selectivity of GPR55.

  • Research Products

    (4 results)

All 2023 2022

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells2023

    • Author(s)
      Nagasaki Shinji C.、Fukuda Tomonori D.、Yamada Mayumi、Suzuki Yusuke III、Kakutani Ryo、Guy Adam T.、Imayoshi Itaru
    • Journal Title

      Cell Structure and Function

      Volume: 48 Pages: 31~47

    • DOI

      10.1247/csf.22074

    • Peer Reviewed / Open Access
  • [Presentation] Functional analyses of lysophospholipid/GPR55 signalling in neuronal circuit development2023

    • Author(s)
      Adam T. Guy
    • Organizer
      20th Joint Mini-Symposium in Cell and Molecular Biology, National Taiwan University
    • Invited
  • [Presentation] Radial Glial secreted phospholipase A2 group V enzyme modulates LysoPtdGlc/GPR55-mediated nociceptive axon guidance in spinal cord development2022

    • Author(s)
      Adam T. Guy
    • Organizer
      Cold Spring Harbor Laboratory Meeting "Molecular Mechanisms of Neuronal Connectivity"
    • Int'l Joint Research
  • [Presentation] Secreted phospholipase A2 group V expressed by radial glia modulates LysoPtdGlc/GPR55-mediated nociceptive axon guidance in spinal cord development2022

    • Author(s)
      Adam T. Guy
    • Organizer
      Japan Neuroscience Society Meeting "Neuro2022"

URL: 

Published: 2023-12-25  

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