Development of evaluation model of intestinal damage with human iPS cell-derived intestinal cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K07034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47030:Pharmaceutical hygiene and biochemistry-related
• Research Institution: Nagoya City University
• Principal Investigator: Iwao Takahiro 名古屋市立大学, 医薬学総合研究院(薬学), 准教授 (50581740)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 消化管 / 粘膜障害 / iPS細胞

Outline of Final Research Achievements

In this study, we generated the intestinal organoids from human iPS cells and were able to develop the method in which the cells were able to be cultured with maintaining crypt-villus structures by the two-dimensional culture of the intestinal organoids. We could obtain preliminary findings to be suggested that the intestinal mucosal damage could be able to be evaluated by using the two-dimensional cultured cells. Additionally, we were able to develop the differentiation method into the endodermal cells from human iPS cells efficiently and the culture method in which the two-dimensional intestinal organoids can be cultured for a relatively long term with keeping crypt-villus structures.

Free Research Field

医療系薬学

Academic Significance and Societal Importance of the Research Achievements

本研究ではヒトiPS細胞から腸管上皮細胞および腸管オルガノイドを作製し、疾患や医薬品によるの消化管傷害を評価可能なin vitroモデル系を構築することを目的として研究を進めた。その結果、ヒトiPS細胞由来腸管細胞が消化管の粘膜障害を評価するためのin vitroモデル系としての応用の可能性を示唆することができた。したがって、本研究では創薬研究等での利用に向けて有用な知見を得ることができたと考えられた。