Screening for chondrogenic enhancer from microbial origin.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K07115
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47050:Environmental and natural pharmaceutical resources-related
• Research Institution: Kitasato University
• Principal Investigator: Ohte Satoshi 北里大学, 薬学部, 講師 (00547979)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 天然物 / 軟骨分化 / 変形性関節症

Outline of Final Research Achievements

In the present study, we established an assay system to assess chondrogenic differentiation and screened for natural product that promote chondrogenic differentiation to provide lead compounds for the treatment of osteoarthritis with compounds that induce chondrogenic differentiation.In addition to ATDC5 cells, which are commonly used as chondrocytes, we introduced a mouse limb bud-derived cell line, MLB13MYC clone14 cells, and established an assay system. Using the established system, we screened microbial resources and found three fungal-derived compounds with chondrogenic differentiation-promoting activity.

Free Research Field

天然物化学

Academic Significance and Societal Importance of the Research Achievements

軟骨分化を誘導するという新しいメカニズムによる変形性関節症の治療薬リード化合物の提供を目指し、微生物資源を対象にスクリーニングを行った結果、3種の活性化合物を単離・同定した。これらの化合物が軟骨分化を促進するという報告は過去になく、初めての知見であった。今後、これら化合物の詳細な作用機序の解析などから、新たな変形性関節症治療薬リード化合物としての展開が期待される。