Elucidation of the mechanism of cell membrane dynamics and cancer malignant signaling through RAGE transmembrane domain

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K07323
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 48040:Medical biochemistry-related
• Research Institution: Kanazawa University
• Principal Investigator: Munesue Seiichi 金沢大学, 医学系, 助教 (10399040)
• Co-Investigator(Kenkyū-buntansha): 木村 久美 金沢大学, 医学系, 助教 (60409472)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: RAGE / がん悪性化 / 細胞膜ダイナミクス

Outline of Final Research Achievements

The expression of RAGE (receptor for advanced glycation end-products), known as a pattern recognition receptor, is essential for the spheroid formation of human osteosarcoma cells (HOS). In this study, the purpose of this research application was to clarify the intracellular signal for spheroid formation. As a result, we demonstrated that TM (RAGE) localizes to lipid rafts, which are functional domains on the cell membrane, and that the spheroid formation is specific to TM (RAGE). Furthermore, we identified a TM(RAGE)-binding protein X and clarified that protein X is an essential molecule for spheroid formation.

Free Research Field

生化学

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、悪性化骨肉腫細胞が培養下で形成するスフェロイドの形成機構の分子メカニズムの一端を明らかにするものであり、本研究成果に基づいて新たな悪性がんの治療戦略の創出の基礎を生み出そうとするものであり学術的な意義は大きい。
また、日本の死因の第一位はがんであり、がんの治療戦略の創出に関わる研究成果の社会的意義は大きい。