innovative therapeutic approach of some devasting respiratory and cardiac diseases via MFALCs targetting

2021 Fiscal Year Research-status Report

• Project/Area Number: 20K07420
• Research Institution: Hokkaido University
• Principal Investigator: エレワ ヤセル 北海道大学, 獣医学研究院, 助教 (30782221)
• Co-Investigator(Kenkyū-buntansha): 昆 泰寛 北海道大学, 獣医学研究院, 教授 (10178402) ; 市居 修 北海道大学, 獣医学研究院, 准教授 (60547769)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: lung / MFALCs / Bleomycin / Dual effect / Lung Asthma

Outline of Annual Research Achievements

During this fiscal year, I could reveal Dual effect of bleomycin on lung injury and mediastinal fat-associated lymphoid clusters (MFALCs). Also, we could reveal a possible Crosstalk of the Immune Cells within the Lung and MFALCs in papain induced- Lung Asthma-like Mouse Model. Additionally, we started new experiment for the effect of dexamethasone administration on the lung injury and MFALCs.

Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

Due to availability of chemicals from purchased from the previous year

Strategy for Future Research Activity

We are planning to investigate the effect of bleomycin intranasal administration on the lung injury in the lymphoproliferative autoimmune mice model (MRL/Lpr mice) and their control strain (MRL/ MpJ mice). Also, we would like to clarify the ameliorative effect of dexamethasone on the autoimmune lung injury and MFALCs development. Moreover, We are planning to investigate the role of MFALCs in the progression of lung cancer in lung melanoma mice model. Furthermore, we would like to examine the possible immunotherapeutic effect of some lipid nanoparticle targeting immune cells within the MFALCs against lung cancer development.

Causes of Carryover

We will use all the budget to cover publication fees (500000 Yen), Buying chemicals and mice (250000 yen), and attending domestic and international conference (250000 yen).

Research Products

• [Journal Article] Possible Crosstalk of the Immune Cells within the Lung and Mediastinal Fat-Associated Lymphoid Clusters in the Acute Inflammatory Lung Asthma-Like Mouse Model (2021)
  • Author(s): Yaser H A Elewa, Mahmoud Abd Elwakil, Osamu Ichii , Teppei Nakamura , Sherif Kh A Mohamed, Yasuhiro Kon
  • Journal Title: Int J Mol Sci . Volume: 22 (6878) Pages: 1-18
  • DOI: 10.3390/ijms22136878
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dual Effect of Bleomycin on Histopathological Features of Lungs and Mediastinal Fat-Associated Lymphoid Clusters in an Autoimmune Disease Mouse Model (2021)
  • Author(s): Yaser H A Elewa, Osamu Ichii , Teppei Nakamura , Yasuhiro Kon
  • Journal Title: Front Immunol . Volume: 12 Pages: 1-17
  • DOI: 10.3389/fimmu.2021.665100
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Albendazole-loaded cubosomes interrupt the ERK1/2-HIF-1 alpha-p300/CREB axis in mice intoxicated with diethylnitrosamine: A new paradigm in drug repurposing for the inhibition of hepatocellular carcinoma progression (2021)
  • Author(s): Saber S, Nasr M, Saad AS, Mourad AAE, Gobba NA, Shata A, Hafez AM, Elsergany RN, Elagamy HI, El-Ahwany E, Amin NA, Girgis S, Elewa Y.H.A., Mahmoud MH, Batiha GE, Abou El-Rous M, Kamal I, Kaddah MMY, Khodir AE
  • Journal Title: Biomed Pharmacother Volume: 142:112029 Pages: 1-14
  • DOI: 10.1016/j.biopha.2021.112029
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mesenchymal stem cells enhance AQP1 expression in the sublingual salivary gland of ovariectomized menopausal rat model (2021)
  • Author(s): El-naseery N.I., Elewa Y.H.A., Arafa M.A.A., Sabbah W.S., Dessouky A.A
  • Journal Title: ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER Volume: 263: 151714 Pages: 1-9
  • DOI: 10.1016/j.aanat.2021.151714
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Osteoblast-activating peptide exhibits a specific distribution pattern in mouse ovary and may regulate ovarian steroids and local calcium levels (2021)
  • Author(s): Noreldin A., Gewaily M.S. , Is Saadeldin I.M., Abomughaid M. M. , Khafaga A. F. , Elewa Y.H.A.
  • Journal Title: Am J Transl Res Volume: 13(6) Pages: 5796-5814
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Mediastinal fat associated lymphoid clusters: A novel immune structure controlling respiratory diseases and intrathoracic immune hemostasis (2022)
  • Author(s): Y. H. A. Elewa, O. Ichii, T. Nakamura, Y. Kon
  • Organizer: The 5th international Conference of Medical Analysis, Toxins, and IVF”. February 11th, 2022, Damanhur, Behera, Egypt. Keynote speaker.
  • Int'l Joint Research / Invited
• [Presentation] Ameliorative effect of dexamethasone on the development of autoimmune lung injury and mediastinal fat-associated lymphoid clusters in autoimmune disease mouse model (2021)
  • Author(s): Y. H. A. Elewa, Md. Abdul Masum, O. Ichii, T. Nakamura, Sherif Kh. A. Mohamed, Y. Kon.
  • Organizer: The 164 Japanese Association of Veterinary Anatomists (JAVA). (Oral Presentation). Hokkaido University, Japan. September 7-13, 2021