2022 Fiscal Year Final Research Report
Studies for practical application of diagnostic methods for fungal infections using the functionally modified glycoside hydrolase
| Project/Area Number |
20K07487
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 深在性真菌症 / β-D-グルカン / β-1,6-グルカン / β-1,6-グルカナーゼ |
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| Outline of Final Research Achievements |
The present study was carried out to determine the optimal pretreatment method for human serum specimen using in the beta-1,6-glucan specific ELISA-like test. The results showed that the dilution heating method is a suitable method for reducing the influence of factors that inhibit the assay. Furthermore, we found that blood beta-1,6-glucan levels in mice were not increased by a beta-1,6-glucan-containing diet. These results indicate that this assay may be able to specifically detect beta-1,6-glucan released by fungi during fungal infection.
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| Free Research Field |
糖質科学
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| Academic Significance and Societal Importance of the Research Achievements |
酵素をもとに開発したβ-1,6-グルカン高感度検出法によって、これまで解析が困難だったβ-1,6-グルカンの生体内での挙動を追跡できる可能性があった。本研究では、血清などの生体検体中のβ-1,6-グルカンを本測定法で高感度に検出するための最適な前処理法を決定し、感染症診断薬としての応用可能性を示した。また、食事由来β-1,6-グルカンのマウス体内での挙動についての知見はこれまでにもほとんど得られていなかったため、本研究成果は多糖の挙動に関する基礎的な知的基盤としての価値も持つ。
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