2022 Fiscal Year Final Research Report
Development of VLP vaccines using protein-protein self-joining system
| Project/Area Number |
20K07524
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 49060:Virology-related
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中平 洋一 茨城大学, 農学部, 准教授 (40423868)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | ウイルス様中空粒子 / VLP / BVDV / RGNNV / SpyTag/SpyCatcher |
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| Outline of Final Research Achievements |
Bovine viral diarrhea virus (BVDV) is a pathogen of commercial consequence in cattle. Although many vaccines are commercially available, their drawbacks precipitate the need for new effective vaccines. Virus-like particles (VLPs) are safe and powerful technology; however, it is difficult to produce large amounts of BVDV VLP. In this study, we generated red-spotted grouper nervous necrosis virus (RGNNV) VLP presenting BVDV E2 protein by exploiting SpyTag/SpyCatcher technology. Mice immunized with RGNNV VLP conjugated with E2 proteins of Nose and KZ-91-CP strains at day 0 and 14 elicited robust IgG titers against E2 proteins of homologous strains. In addition, this prime-boost regimen induced strong neutralization titers against homologous BVDV strains. Our results indicate that conjugation of E2 protein to RGNNV VLP strongly enhanced the antigenicity of E2 protein and that RGNNV VLPs presenting E2 protein are promising BVDV vaccine candidates.
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| Free Research Field |
ウイルス学
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| Academic Significance and Societal Importance of the Research Achievements |
BVDVは牛ウイルス性下痢・粘膜病を引き起こす重要な病原体で、畜産業に甚大な経済損失をもたらしている。現在、BVDVに対する治療法はなく、感染牛の摘発淘汰またはワクチンによる予防が有効な対策とされている。しかし、現行の生ワクチンは、妊娠牛への接種により胎内感染が起こるため接種ができないという問題がある。また不活化ワクチンは、抗体価が低く予防効果が完全ではないという課題がある。本研究で作成したE2抗原を提示するVLPワクチンは有望なワクチン候補と考えられる。
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