2022 Fiscal Year Final Research Report
Identification of factors highly expressed in three-dimensional cultured cancer cells and expansion to elucidation of tumorigenesis mechanisms
| Project/Area Number |
20K07583
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 50010:Tumor biology-related
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| Research Institution | Osaka Metropolitan University (2022)
Osaka City University (2021)
Yamagata University (2020) |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | がん / モノクローナル抗体 / 三次元細胞培養 / サイトケラチン / 解糖系酵素 / 細胞接着因子 |
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| Outline of Final Research Achievements |
In this study, we found that the antigens of monoclonal antibodies (mAb) 2H7, 7D6 and 7E11, which were established by immunizing 3D-cultured colon cancer cell line DLD-1, were the cytoskeleton molecule CK18, glycolytic enzyme GPI and adherens junction component molecule IQGAP1. CK18 was found to be degraded by cell death induced by 3D culture or by treatment with some anticancer drugs. Furthermore, we showed that GPI expression was increased and the glycolytic system was upregulated in 3D-cultured cancer cells. The enhancement of the glycolytic system in 3D-cultured cancer cells may be caused by hypoxia.
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| Free Research Field |
細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究における3D培養がん細胞の細胞死の誘導および解糖系の亢進は，研究代表者が用いた3D培養大腸がん細胞が生体内の腫瘍のがん細胞の特性を再現し，腫瘍形成メカニズム解明のためのモデルとなると示した。また，抗がん剤誘導した大腸がん細胞におけるCK18の分解が抗がん剤の種類により異なった点について，それぞれの抗がん剤が誘導する細胞死誘導メカニズムに違いがあると示唆され，今後の詳細なメカニズムの解明は抗がん剤の副作用軽減につながると考えられる。
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