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2022 Fiscal Year Final Research Report

Development of novel diagnostic system using exsomes

Research Project

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Project/Area Number 20K07817
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 52010:General internal medicine-related
Research InstitutionTokyo Metropolitan Geriatric Hospital and Institute of Gerontology

Principal Investigator

Ito Masafumi  地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (80393114)

Co-Investigator(Kenkyū-buntansha) 川上 恭司郎  地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (90589227)
水谷 晃輔  岐阜大学, 医学部附属病院, 准教授 (80397356)
Project Period (FY) 2020-04-01 – 2023-03-31
Keywordsエクソソーム / 細胞外小胞 / アルツハイマー病 / 診断法
Outline of Final Research Achievements

The purpose of this study was to purify exosomes from serum and identify novel biomarkers for Alzheimer's disease (AD) by proteomic analysis. Sera from AD patients and healthy subjects were used as samples. (1) Proteomic analysis of exosomes purified by the phosphatidylserine affinity method using stable isotope labeling identified 15 proteins that were significantly increased or decreased in AD patients. (2) Proteomic analysis of exosomes purified by antibodies against exosome marker proteins using label-free strategy identified 13 proteins that were significantly increased or decreased in AD patients. We will validate the significance of these selected biomarker candidates and develop a novel serum exosome-based diagnostic system for AD.

Free Research Field

内科学、分子生物学

Academic Significance and Societal Importance of the Research Achievements

超高齢社会を迎えているわが国では認知症の早期診断は喫緊の課題である。本研究では、血清中のエクソソームに焦点を当て、アルツハイマー病の新規バイオマーカーの探索を行った。本研究により、アミロイドβ、リン酸化タウなど既知のバイオマーカー以外の候補タンパク質を同定した。本研究を発展させることにより、将来的には血清中のエクソソームを用いたアルツハイマー病の診断が可能になることが期待される。

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Published: 2024-01-30  

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