2022 Fiscal Year Final Research Report
Molecular mechanisms and prevention methods of Alzheimer's disease
| Project/Area Number |
20K07863
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 52020:Neurology-related
|
|---|
| Research Institution | Kanazawa Medical University (2022)
Kanazawa University (2020-2021) |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
山田 正仁 金沢大学, 医学系, 協力研究員 (80191336)
小野 賢二郎 金沢大学, 医学系, 教授 (70377381)
|
|---|
| Project Period (FY) |
2020-04-01 – 2023-03-31
|
| Keywords | Alzheimer病 / アミロイドβ蛋白質 / 個体間伝播 / 医療行為 / 防御法 |
|---|
| Outline of Final Research Achievements |
The objective of this study is to develop a method to prevent human-to-human transmission of Alzheimer's disease (AD) pathological changes due to medical intervention. In in vitro aggregation studies of Aβ aggregates with synthetic Aβ1-40 and Aβ1-42 peptides, autoclaving at 135 °C for 120 minutes was required to inactivate the seeding activities of both Aβ1-40 and Aβ1-42 aggregates was necessary. Human autopsy brain homogenate was inactivated by autoclaving at 135 °C for 120 minutes, and the inactivated brain homogenate was inoculated into AD model mouse brains. However, Aβ deposition was found in the brain of inoculated AD model mouse, which suggested that autoclaving may not be effective to prevent transmission of Aβ pathology among individuals.
|
| Free Research Field |
脳神経内科学
|
| Academic Significance and Societal Importance of the Research Achievements |
試験管内でAβseeding活性を不活化させるオートクレーブ条件を世界で初めて発見した。この条件は通常の医療機関で用いるオートクレーブ条件よりも高温で長時間であった。この条件で処理したヒトAD剖検脳ホモジネートをADモデルマウスでAβseeding活性を確認したが、生体内ではAβseeding活性が不活化出来ていなかった。AD病理学的変化のヒトからヒトへの伝播を予防するためには、オートクレーブ以外の方法を検討する必要があると考えられた。今後、AD病理学的変化のヒトからヒトへの伝播を予防する方法を開発するための研究を継続する必要がある。
|
