2022 Fiscal Year Final Research Report
Mapping of abnormal neural circuit formation by diffusion tensor MRI and 3D construction of confocal microscope images
| Project/Area Number |
20K08089
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 52040:Radiological sciences-related
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
桝 正幸 筑波大学, 医学医療系, 教授 (20243032)
桝 和子 筑波大学, 医学医療系, 講師 (50344883)
鎌形 康司 順天堂大学, 医学部, 准教授 (60568153)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | endosulfatase / heparan sulfate / MRI / axon guidance |
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| Outline of Final Research Achievements |
We mapped neural circuit dysplasia by next-generation diffusion MRI analysis including diffusion tensor and three-dimensional construction of confocal images of transparent brain. By combining the innovative brain clearing technology and cutting-edge MRI technology proposed in recent years, this research has not only elucidated the function of sulfatase in axon guidance, but also a new neuropsychiatric disorder with minute structural abnormalities.
We reported impaired commissural fibers in the brains of Sulf1/2 DKO mice using radiological and histological methods. Using fiber tractography analysis, we found abnormalities in the commissural fiber system of the corpus callosum and dorsal hippocampal commisure and confirmed these histological abnormalities by FluoroMyelin staining and anterograde tracing of the DHC fibers. These results indicate that Sulf1/2 play an important role in the normal development of commissural fibers.
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| Free Research Field |
Neurosciences
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| Academic Significance and Societal Importance of the Research Achievements |
This study not only elucidated the role of Sulf1/2 in commissure formation but also contributed to the search for new surrogate markers for psychiatric and neurological disorders associated with structural abnormalities.
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