2022 Fiscal Year Final Research Report

Development of fetal therapy of congenital diaphragmatic hernia by intra-amniotic injection of exosomes derived from mesenchymal stem cells

Research Project

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Project/Area Number	20K08188
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 52050:Embryonic medicine and pediatrics-related
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	Furukawa Taizo 京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (20515291)
Co-Investigator(Kenkyū-buntansha)	松田修京都府立医科大学, 医学(系)研究科(研究院), 教授 (00271164) 岸田綱郎京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00370205) 東真弓京都府立医科大学, 医学(系)研究科(研究院), 助教 (10380453) 馬庭淳之介国立研究開発法人国立がん研究センター, 中央病院, 医員 (20847222) 坂井宏平京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (30515292) 田尻達郎九州大学, 医学研究院, 教授 (80304806)
Project Period (FY)	2020-04-01 – 2023-03-31
Keywords	間葉系幹細胞 / エクソソーム / 先天性横隔膜ヘルニア / 羊水腔投与 / ラット
Outline of Final Research Achievements	For congenital diaphragmatic hernia (CDH), with the ultimate goal of developing a new treatment, tracheal occlusion (TO), which has already been clinically applied using medium animals (rabbit), was performed, and MSC-derived EVs were administered. It was planned to conduct a study on treatment effects. TO on fetal rabbits under intravenous anesthesia resulted in a stable success rate. On the other hand, in the MSC experiment, we used rat lung buds, which we have used before, to confirm the difference in the effects of direct and paracrine actions of MSCs before extracting exosomes. No significant difference was observed, and it was not possible to proceed to exosome extraction.
Free Research Field	小児外科
Academic Significance and Societal Importance of the Research Achievements	CDHの胎児治療において、TOは生存率は49％と救命率はまだ低く、新規胎児治療開発が急務である。今回の実験では、MSCの持つ直接作用、paracrine作用の効果の違いについては有意差は認めなかったものの、肺成熟効果を保つ事は確実であり、今後の新規治療法開発に向けて期待のできる細胞ソースであると思われた。