2021 Fiscal Year Research-status Report
Investigation of tumor immune escape mechanisms in liver cancer using allograft mouse model
| Project/Area Number |
20K08276
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| Research Institution | Chiba University |
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Principal Investigator |
千葉 哲博 千葉大学, 大学院医学研究院, 講師 (00381583)
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| Co-Investigator(Kenkyū-buntansha) |
金山 健剛 千葉大学, 医学部附属病院, 医員 (20835102)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 腫瘍微小環境 |
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| Outline of Annual Research Achievements |
同系マウスの皮下へ移植（同種異所性移植モデル）による腫瘍微小環境の免疫学的解析を行目的とし、Hepa1-6細胞のC57BL/6マウス、H22細胞のBalb/cマウスへの皮下移植の系を作成した。移植後数週間にわたって担癌マウスの腫瘍増大をモニターするとともに、腫瘍組織をコラゲナーゼ分離により分散し、比重遠心法および磁気細胞分離法により腫瘍細胞、血管内皮細胞、免疫担当細胞（細胞障害性T細胞、制御性T細胞、NK細胞、M2マクロファージなど）の単離ができるようになった。さらにフローサイトメトリーを用いてこれらの腫瘍組織内の各細胞分画の詳細な解析を行った。
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
COVID-19の影響により海外からの試薬類などの購入に時間を要したため、多少の遅れが生じている。
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| Strategy for Future Research Activity |
腫瘍微小環境の評価として、サイトカインアレイを用いてIFN-γ, TNF-alpha、VEGFなどのサイトカイン濃度を網羅的に測定する。また、マルチキナーゼ阻害剤、VEGF阻害剤、EZH2阻害剤を単独投与または免疫チェックポイント阻害剤と併用投与し、抗腫瘍効果を検討するとともに、微小環境構成細胞の変化を評価する。
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| Causes of Carryover |
物品費が予想より若干少なかったため、1091円の次年度使用額が生じた。比較的少額であり、次年度の予算と合わせて問題なく執行予定である。
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[Journal Article] EZH1/2 inhibition augments the anti-tumor effects of sorafenib in hepatocellular carcinoma.2021
Author(s)
Kusakabe Y, Chiba T, Oshima M, Koide S, Rizq O, Ao J, Kaneko T, Kanzaki H, Kanayama K, Maeda T, Saito T, Nakagawa R, Kobayashi K, Kiyono S, Nakamura M, Ogasawara S, Suzuki E, Nakamoto S, Yasui S, Mikata R, Muroyama R, Kanda T, Maruyama H, Kato J, Mimura N, Ma A, Jin J, Zen Y, Otsuka M, Kaneda A, Iwama A, Kato N.
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Journal Title
Sci Rep.
Volume: 11
Pages: 21396
DOI
Peer Reviewed / Open Access
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[Journal Article] Human hepatocyte-derived extracellular vesicles attenuate the carbon tetrachloride-induced acute liver injury in mice2021
Author(s)
Kakizaki M, Yamamoto Y, Nakayama S, Kameda K, Nagashima E, Ito M, Suyama T, Matsuzaki Y, Chiba T, Sumiyoshi H, Inagaki Y, Kotani A.
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Journal Title
Cell Death Dis.
Volume: 12
Pages: 1010
DOI
Peer Reviewed / Open Access
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[Journal Article] Genetic profiles of Barrett's esophagus and esophageal adenocarcinoma in Japanese patients2021
Author(s)
Tokunaga M, Okimoto K, Akizue N, Ishikawa K, Hirotsu Y, Amemiya K, Ota M, Matsusaka K, Nishimura M, Matsushita K, Ishikawa T, Nagashima A, Shiratori W, Kaneko T, Oura H, Kanayama K, Ohta Y, Taida T, Saito K, Matsumura T, Chiba T, Mochizuki H, Arai M, Kato J, Ikeda JI, Omata M, Kato N.
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Journal Title
Sci Rep.
Volume: 11
Pages: 17671
DOI
Peer Reviewed / Open Access
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[Journal Article] Diagnostic value of IMP3 and p53 immunohistochemical staining in EUS-guided fine-needle aspiration for solid pancreatic tumors2021
Author(s)
Mikata R, Yasui S, Kishimoto T, Kouchi Y, Shingyoji A, Senoo J, Takahashi K, Nagashima H, Kusakabe Y, Ohyama H, Ohno I, Sugiyama H, Chiba T, Kato J, Kato N.
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Journal Title
Sci Rep.
Volume: 11
Pages: 17257
DOI
Peer Reviewed / Open Access
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[Journal Article] Comprehensive mutational analysis of background mucosa in patients with Lugol-voiding lesions2021
Author(s)
Akizue N, Okimoto K, Arai M, Hirotsu Y, Amemiya K, Oura H, Kaneko T, Tokunaga M, Ishikawa K, Ohta Y, Taida T, Saito K, Maruoka D, Matsumura T, Nakagawa T, Nishimura M, Chiba T, Matsushita K, Mochizuki H, Yokosuka O, Omata M, Kato N.
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Journal Title
Cancer Med.
Volume: 10
Pages: 3545-3555
DOI
Peer Reviewed / Open Access
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[Journal Article] Analysis of circulating cell-free DNA after endoscopic ultrasound-guided fine needle aspiration in pancreatic ductal adenocarcinoma2021
Author(s)
Asano K, Mikata R, Chiba T, Kan M, Maruta S, Yamada T, Miura Y, Shima Y, Sensui M, Nagashima H, Yokoyama M, Ohyama H, Kusakabe Y, Yasui S, Sugiyama H, Ohno I, Kato J, Takano S, Ohtsuka M, Kato N.
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Journal Title
Pancreatology.
Volume: S1424
Pages: 3903
DOI
Peer Reviewed
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[Journal Article] Serum Angiopoietin 2 acts as a diagnostic and prognostic biomarker in hepatocellular carcinoma2021
Author(s)
Ao J, Chiba T, Kanzaki H, Kanayama K, Shibata S, Kurosugi A, Iwanaga T, Kan M, Sakuma T, Qiang N, Ma Y, Kojima R, Kusakabe Y, Nakamura M, Kobayashi K, Kiyono S, Kanogawa N, Saito T, Nakagawa R, Kondo T, Ogasawara S, Suzuki E, Nakamoto S, Muroyama R, Tawada A, Kato J, Kanda T, Maruyama H, Kato N.
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Journal Title
J Cancer.
Volume: 12
Pages: 2694-2701
DOI
Peer Reviewed / Open Access
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[Journal Article] Acquisition of mesenchymal-like phenotypes and overproduction of angiogenic factors in lenvatinib-resistant hepatocellular carcinoma cells2021
Author(s)
Ao J, Chiba T, Shibata S, Kurosugi A, Qiang N, Ma Y, Kan M, Iwanaga T, Sakuma T, Kanzaki H, Kanayama K, Kojima R, Kusakabe Y, Nakamura M, Saito T, Nakagawa R, Kondo T, Ogasawara S, Suzuki E, Muroyama R, Kato J, Mimura N, Kanda T, Maruyama H, Kato N.
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Journal Title
Biochem Biophys Res Commun.
Volume: 549
Pages: 171-178
DOI
Peer Reviewed
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[Journal Article] The impact of FGF19/FGFR4 signaling inhibition in antitumor activity of multi-kinase inhibitors in hepatocellular carcinoma2021
Author(s)
Kanzaki H, Chiba T, Ao J, Koroki K, Kanayama K, Maruta S, Maeda T, Kusakabe Y, Kobayashi K, Kanogawa N, Kiyono S, Nakamura M, Kondo T, Saito T, Nakagawa R, Ogasawara S, Suzuki E, Ooka Y, Muroyama R, Nakamoto S, Yasui S, Tawada A, Arai M, Kanda T, Maruyama H, Mimura N, Kato J, Zen Y, Ohtsuka M, Iwama A, Kato N.
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Journal Title
Sci Rep.
Volume: 11
Pages: 5303
DOI
Peer Reviewed / Open Access
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[Journal Article] The combination of the tubulin binding small molecule PTC596 and proteasome inhibitors suppresses the growth of myeloma cells2021
Author(s)
Nagai Y, Mimura N, Rizq O, Isshiki Y, Oshima M, Rizk M, Saraya A, Koide S, Nakajima-Takagi Y, Miyota M, Chiba T, Oshima-Hasegawa N, Muto T, Tsukamoto S, Mitsukawa S, Takeda Y, Ohwada C, Takeuchi M, Iseki T, Nakaseko C, Lennox W, Sheedy J, Weetall M, Yokote K, Iwama A, Sakaida E.
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Journal Title
Sci Rep.
Volume: 11
Pages: 2074
DOI
Peer Reviewed / Open Access
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