2022 Fiscal Year Final Research Report
A study for intestinal archaea in human health and disease
| Project/Area Number |
20K08384
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 53010:Gastroenterology-related
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
玉木 秀幸 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (00421842)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | archaea / colitis / irritable bowel syndrome / gut microbiota / mucosal immnology |
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| Outline of Final Research Achievements |
The aim of this study is to characterize human intestinal archaea, examine their effects on inflammatory bowel disease (IBD), and attempt to culture and functionally-analyze unknown archaea. During the study period, we (Shimane university) have completed sampling from more than 100 IBD patients. At the National Institute of Advanced Industrial Science and Technology, we have carefully investigated and tried to analyze with new microbial detection methods and culturing techniques. With these new methods, we successfully detected and cultured some archaea species from the mouse intestine as a preliminary experiment. However, the amount of microbial DNA in the human biopsy specimens was too small to be further analyzed. Thus, another attempt using new DNA amplification technology is currently in progress.
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| Free Research Field |
microbiota
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では最新の高性能な機器や試薬を用いて、消化管に生息する未知の微生物の検討を重ねてきた。しかし、そのDNA抽出と培養過程が想定以上に困難であり、微生物解析・培養法のさらなる進化が必要と感じられた。現在、マウスを用いた予備実験で使用した方法をさらに進化させる検討が進行しており、うまくいけばヒトサンプルの解析に入る予定である。これにより炎症性腸疾患に対する消化管アーキアの役割の解明と、その経路を介した治療応用につながる可能性があると考える。
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