2023 Fiscal Year Final Research Report
Establishment and application of proteome analysis methods for endomyocardial biopsy samples
| Project/Area Number |
20K08485
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 53020:Cardiology-related
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
Wakabayashi Masaki 国立研究開発法人国立循環器病研究センター, オープンイノベーションセンター, 室長 (70552024)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | 心内膜心筋生検 / プロテオミクス |
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| Outline of Final Research Achievements |
In this study, we established a proteomic and post-translational modification analysis method for the analysis of endomyocardial biopsy samples, and attempted to apply the method to human tissues. However, we were unable to obtain data that would allow us to analyze pathological conditions, taking into account sample aging, individual differences, and differences in expression derived from the site of removal. Although securing the number of samples and standardization of sample collection protocols are issues to be addressed, it is expected that the analysis method for myocardial biopsy tissue established in this study will serve as a platform that can be applied to the analysis of cardiac diseases in general.
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| Free Research Field |
プロテオミクス
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| Academic Significance and Societal Importance of the Research Achievements |
ヒトの診断・治療への応用を鑑みれば、ヒト試料の解析は研究者にとって最重要課題である。しかしながら、心疾患を対象とした研究においては、利用できるヒト組織試料が、剖検心や移植に伴う摘出心に制限されるため、病態の進行や回復を反映した時系列情報の取得は難しい。この障壁を乗り越えて様々な心疾患研究を推進可能な方法として、診療のために取得した心内膜心筋生検試料の残余検体の利用が挙げられる。本研究はこの残余検体を対象として分子レベルでの詳細な解析を可能とする手法を確立・提案するものであり、心疾患の研究領域において非常に意義深い研究と言える。
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