Research on pathological diagnosis, genetic analysis, and therapeutic efficacy using patient-derived organoids

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K08574
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53030:Respiratory medicine-related
• Research Institution: University of Yamanashi (2022-2023); Keio University (2020-2021)
• Principal Investigator: Ikemura Shinnosuke 山梨大学, 大学院総合研究部, 特任講師 (30445291)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: オルガノイド培養 / 肺癌 / 病理学的診断 / 分子生物学的診断 / 精密医療

Outline of Final Research Achievements

We have generated lung cancer organoid libraries derived from patients with diverse molecular and histological phenotypes of lung cancer (Ebisudani T et al. Cell Rep. 2023 Mar 28;42(3):112212.). We established 43 lung cancer organoid libraries covering adenocarcinoma, squamous cell carcinoma, small cell carcinoma, and large cell neuroendocrine carcinoma. Organoids could also be established from sputum and circulating tumor cells in blood. Organoids were transplanted into immunodeficient mice, and their pathology was compared with that of the respective patients' tumors, which matched in pathological findings, genetic mutations, copy number aberrations, aneuploidy, and protein expression.

Free Research Field

肺癌

Academic Significance and Societal Importance of the Research Achievements

今回確立した肺癌患者由来のオルガノイド樹立技術の応用により、臨床的な薬剤感受性の予測、薬剤耐性化機序の解明、予後予測などが可能となる。また、基礎研究として、発癌・その予防・抑制機序の解明、薬剤開発などに広く応用することが可能である。肺癌だけでなく、広く癌研究、癌治療への応用も期待される。