Development of an innovative differential diagnosis method for diphyllobothriasis and taeniasis

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K08816
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 54030:Infectious disease medicine-related
• Research Institution: Asahikawa Medical College
• Principal Investigator: Sako Yasuhito 旭川医科大学, 医学部, 教授 (40312459)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 裂頭条虫 / テニア条虫 / 鑑別診断 / POCテスト

Outline of Final Research Achievements

In this study, differential diagnosis methods of parasites in clinical and endemic fields were developed. Differential diagnostic methods developed combines a differential DNA detection method for three Dibothriocephalus species and three Taenia species using the LAMP method with a method for differential detection of amplified DNA by ICT. The species-specific DNA amplification test using the LAMP method targeted the mitochondrial gene COX1. In addition, the 5' end of the primer was labeled with FITC, biotin, and DIG to enable differential detection of amplified DNA by ICT. Accuracy analyses by using Dibothriocephalus DNA, Taenia DNA, and other parasite DNA revealed that the differential diagnosis methods developed in this study have high specificity.

Free Research Field

寄生虫学

Academic Significance and Societal Importance of the Research Achievements

寄生虫を鑑別診断することは「臨床と公衆衛生」にとって非常に重要である。本研究課題で開発した鑑別診断法は寄生虫検査の経験のない人でも迅速かつ簡便に寄生虫を鑑別診断でき、さらに社会実装できる高感度なpoint-of-careテスト（POCT）である。また、本研究の成果はウイルス、細菌、原虫、吸虫、線虫などの感染症の、汎用性の高い簡便で迅速な病原体同定法の開発に容易に応用でき、社会に与えるインパクトは大きい。