2022 Fiscal Year Final Research Report
Development of a rapid and easy genotyping method for identification and monitoring transmission events by hypervirulent Klebsiella pneumoniae
Project/Area Number |
20K08819
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 54030:Infectious disease medicine-related
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Research Institution | Nagoya University |
Principal Investigator |
Kawamura Kumiko 名古屋大学, 医学系研究科(保健), 准教授 (30335054)
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Co-Investigator(Kenkyū-buntansha) |
八木 哲也 名古屋大学, 医学系研究科, 教授 (70333573)
鈴木 匡弘 藤田医科大学, 医学部, 准教授 (70446649)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 高病原性肺炎桿菌 / カルバペネマーゼ産生腸内細菌目細菌 / multiplex PCR法 / 菌種マーカー / sequence type / 莢膜型K1 / 病原遺伝子(rmpA/A2) / 薬剤耐性遺伝子 |
Outline of Final Research Achievements |
We aimed to develop the multiplex PCR typing method for K. pneumoniae that would be useful in hospital epidemiology and outbreak investigation. This typing method consists of two multiplex PCR reactions that were designed to detect 25 ORFs specific to bacterial genetic lineages, species, antimicrobial-resistant genes (blaCTX-M, blaIMP and blaKPC), a capsular K1-specific gene and a virulence factor gene (rmpA/A2). The distribution patterns of ORFs among K. pneumoniae correlated well with multilocus sequence typing, and closely related species could be distinguished and antimicrobial resistance and hypervirulence genes were identified. This typing method is a rapid and easy genotyping method for monitoring transmission events by K. pneumoniae in clinical microbiology laboratories, and supplies clear and informative molecular typing results for the isolates. In addition, this would facilitate molecular epidemiological analysis in infection control and hospital epidemiology investigations.
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Free Research Field |
臨床微生物学
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Academic Significance and Societal Importance of the Research Achievements |
本研究で構築したmultiplex PCR typing methodは、病原性やアウトブレイクと関連する遺伝子型や莢膜型(K1)、薬剤耐性遺伝子および病原遺伝子の保有をOne-stepで解析する分子疫学解析法であり、分子疫学解析標準法と同等の性能を有していた。日常検査における本typing methodの導入は、肺炎桿菌とその近縁種の分布、カルバペネム耐性株および高病原性株の流布の実態を明らかにするとともに、日常診療におけるそれら菌株識別の有用性を明らかにすることに貢献するものと考える。加えて、それらの結果は病院の感染制御および院内感染への対策強化にも貢献することが期待される。
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