2022 Fiscal Year Final Research Report
Fundamental analysis based on protein processing for dengue vaccine development
| Project/Area Number |
20K08822
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 54030:Infectious disease medicine-related
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | iPS cell / dengue / protein processing / liver cells / dendritic cells |
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| Outline of Final Research Achievements |
In this study, we aimed to identify virus-derived peptide patern that induce effective cellular immunity against dengue virus infection by comparing peptide sequences recovered from virus-infected hepatocytes and dendritic cells.
At that time, it took time to construct an experimental system sufficient for preparation of a large number of hepatocytes necessary for experiments, unlike other cells which information was already obtained. As a result, we constructed a new establishment method including freeze-thaw during the preparation, unlike the conventional method.
Infection and analysis of the results using dengue virus were not conducted by the end of the period, so this project will be continued with other budgets. The constructed new liver cell line induction method is useful for research on many liver diseases. Therefore, we believe that our study will contribute to wide range of researches.
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| Free Research Field |
Immunology
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| Academic Significance and Societal Importance of the Research Achievements |
当初の目的であったデング熱に対して有効な細胞性免疫を誘導するペプチド配列（エピトープ）パターンの同定には、計画期間中に至ることが出来なかった。
一方、副産物としてではあるが、iPS細胞からの新たな肝細胞株樹立方法を構築し、論文等による成果公表を準備中である。これは、デング熱のみならず肝臓が感染部位となる多くの感染症を含む疾患の研究に活用可能な技術であり、学術的意義と社会的な意義を有すると考える。
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