Analysis of role of exosome in septic peritonitis and its clinial apllication

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09052
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: University of Yamanashi
• Principal Investigator: Kono Hiroshi 山梨大学, 大学院総合研究部, 特任准教授 (40322127)
• Co-Investigator(Kenkyū-buntansha): 細村 直弘 山梨大学, 大学院総合研究部, 助教 (60402070)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 臓器マクロファージ / 肝類洞免疫 / 敗血症性臓器障害

Outline of Final Research Achievements

The activation of exosome (Exo) and macrophages in the development of lung injury caused by sepsis was studied. Exo was isolated from blood collected after CLP model and injected intravenously into normal rats. The histopathological changes in the lungs and the number of neutrophils infiltrating the lungs were examined after administration. Hepatic pulmonary macrophages（Mf） were isolated from normal rats, and the activation of Mf by Exo stimulation was examined , and TLR4 receptor antagonist was also examined in the co-cultured system. TLR4 receptor antagonist significantly decreased TNF production. Exo in blood induced acute lung injury in sepsis.

Free Research Field

炎症免疫機構の解析

Academic Significance and Societal Importance of the Research Achievements

血中のエクソゾームが敗血症時の臓器障害の発症に関与する事実が明らかとなり、エクソゾームが敗血症に起因する肺障害発症の基幹的因子である可能性が考えられる。今後、エクソゾームを標的とした敗血症性臓器障害の治療への要用が期待できる。