2022 Fiscal Year Final Research Report
Distribution and clonality of multiple intraductal neoplasms of the pancreas evaluated by morphology
| Project/Area Number |
20K09070
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 55020:Digestive surgery-related
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| Research Institution | Center for Clinical and Biomedical Research, Sapporo Higashi Tokushukai Hospital |
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Principal Investigator |
Karasaki Hidenori 医療法人徳洲会札幌東徳洲会病院医学研究所, がん生物研究部, 客員研究員 (50374806)
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| Co-Investigator(Kenkyū-buntansha) |
水上 裕輔 旭川医科大学, 医学部, 教授 (30400089)
小野 裕介 医療法人徳洲会札幌東徳洲会病院医学研究所, ゲノム診断研究部, 部門長 (40742648)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 背景膵 / 膵管内病変 / clonality / 正常膵管 / 膵発癌 / 領域発癌 / KRAS |
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| Outline of Final Research Achievements |
The liquid biopsy, promising clinical application for early detection of pancreatic neoplasms consist of many molecular alterations about not only the index tumor but also the background. For better understanding of the genetic alterations occurring throughout the pancreas, mutation analysis of multiple sites, including normal pancreatic ducts, in two cases with high-grade IPMN and double pancreatic cancer as index lesions, respectively. Approximately 40% of the patients had some kind of gene mutation. The majority of these mutations were low-grade intraductal lesions, but mutations were also found in some normal pancreatic ducts. There was no significant difference in the number of samples examined between the two cases, the distribution of grades, and the rate of concordance in KRAS mutation type between index lesions and background pancreatic sites, but individual differences were observed in the number of KRAS mutations.
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| Free Research Field |
消化器病学
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| Academic Significance and Societal Importance of the Research Achievements |
難治癌である膵癌の早期発見のために液体生検の臨床応用が期待されている。その解析資料として血液、十二指腸液、膵液などが検討されているが、いずれであっても指標病変だけではなく、背景に起こっている様々な分子情報が含まれることになる。これらの適切な解釈なしに液体生検の臨床応用はありえないが、背景膵の分子変化や、指標病変との関連の詳細は不明な点が多い。本研究は少数例ではあるが正常膵管も含め背景膵の多数箇所と指標病変の複数箇所の変異解析を行い、症例間で共通している、あるいは個体間で異なる変化を模索した。本研究の結果を基に多種類の膵腫瘍の背景情報を類型化することで液体生検の臨床応用に寄与しうる。
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