Effects of Human Amniotic Membrane on Angiogenesis and Wound Healing in a Rat Ischemic Wound Model

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09138
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55030:Cardiovascular surgery-related
• Research Institution: Tohoku University
• Principal Investigator: Ogasawara Hiroyuki 東北大学, 医学系研究科, 大学院非常勤講師 (90868118)
• Co-Investigator(Kenkyū-buntansha): 宮城 重人 東北大学, 大学病院, 准教授 (00420042) ; 齋藤 昌利 東北大学, 医学系研究科, 教授 (00451584) ; 星合 哲郎 東北大学, 大学病院, 講師 (10569560) ; 戸子台 和哲 東北大学, 大学病院, 准教授 (50581641) ; 菅原 宏文 東北大学, 医学系研究科, 大学院非常勤講師 (60451572)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ヒト羊膜 / 血管新生促進作用 / 創傷治癒 / 虚血性創傷

Outline of Final Research Achievements

Using a rat model of abdominal ischemic wounds, we examined the effect of human amniotic membrane on wound healing in ischemic wounds. Comparison was made between two groups: "amniotic membrane transplantation group" with human amniotic membrane and "control group" without amniotic membrane. After surgery, the area of the wound was measured and the wound healing rate was compared between the two groups. The mechanism of the wound healing promotion effect was examined from the three viewpoints of epithelialization promotion, angiogenesis promotion, and anti-inflammation. As a result, the wound healing promotion effect of using human amniotic membrane was confirmed, and significant results were also obtained for the angiogenesis promotion effect. On the other hand, the anti-inflammatory effect was not clarified in this study, suggesting that human amniotic membrane may promote healing of ischemic wounds by enhancing angiogenesis.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

新鮮ヒト羊膜の虚血性創傷に対する血管新生促進作用・創傷治癒促進作用を検証し、新鮮ヒト羊膜が虚血性創傷に対する有用な創傷被覆剤の1つになる可能性を明らかにした。これまで熱傷、皮膚欠損など非虚血性創傷におけるヒト羊膜の創傷治癒や血管新生の有効性が報告され、虚血性創傷に対しても創傷被覆剤として応用できる可能性は高いと考えられる。