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2021 Fiscal Year Research-status Report

敗血症臓器障害におけるVEGFとアンジオポエチンの研究:短期型βブロッカーの作用

Research Project

Project/Area Number 20K09282
Research InstitutionIbaraki Prefectural University of Health Science

Principal Investigator

河野 了  茨城県立医療大学, 付属病院, 教授 (90323295)

Co-Investigator(Kenkyū-buntansha) 下條 信威  筑波大学, 医学医療系, 講師 (20462210)
Project Period (FY) 2020-04-01 – 2023-03-31
KeywordsVEGF / アンジオポエチン / ランジオロール
Outline of Annual Research Achievements

We also extended the research on sepsis-induced ALI (acute lung injury) in the 2nd year. In the 2nd year, we investigated whether landiolol hydrochloride, an ultra-short-acting β-blocker, has any vital role in lessening LPS-induced ALI (acute lung injury) by altering the angiopoietin-2. Indeed, angiopoietin-2 is an endothelium-specific growth factor and is regulated by proinflammatory stimuli with a destabilization of vascular endothelium and an increase in vascular leakage. We used male Wistar rats at 8weeks of age and were administered with either saline or lipopolysaccharide (LPS) for three hours (3h), and some of the LPS-administered rats were continuously treated with landiolol for 3h. This experimental setting has already validated LPS-induced ALI with significant morphological alteration in the lung, upregulated both circulatory and pulmonary TNF-α and IL-6, and upregulation of pulmonary level of angiopoietin-2. These changes accompanied an LPS-induced considerable decrease in pulmonary levels of VEGF with Pa02. Here we found that the treatment of LPS-administered rats with landiolol for 3h significantly stopped the progression of ALI with marked normalization of angiopoietin-2. This treatment also considerably normalized blood gas parameters, and pulmonary downregulated VEGF levels. Collectively, our data indicate that landiolol treatment in LPS-administered rats plays an essential role in attenuating ALI through modulation of angiopoietin-2 with concomitant VEGF signaling reversal.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

We are on the planned activities of the research with the target milestone. The current study is almost on the right track, both from the context of the timeline and the number of research activities scheduled to be done to properly manage the project budget and research facilities available for the first two years. We did not face any technical difficulties. Although Covid has impacted many things, we were careful to perform every target of the project with excellent efficiency and impact. We could have all reagents available to conduct the studies. We could reproduce data in first and second years of the experiment. All experiments were based on time-course and dose-dependent studies. Thus the time point and the dose were validated in both of these years for the experimental setting.

Strategy for Future Research Activity

In the 3rd year of the project, we will continue the investigation of VEGF and angiopoietin system in various organs in sepsis animals besides lung tissues. Heart, kidney, liver, and brain are essential to be investigated. The effects of landiolol treatment will also be simultaneously explored. We will also aim to use Tie-2 receptor knockout mice in the current experimental setting with sepsis induction and examine the effects of landiolol treatment.

Causes of Carryover

新型コロナウイルス感染症の蔓延のため、予定していた学会に参加できず、旅費が使用できなかった。次年度は、様子を見て参加できるなら旅費を使用する予定である。

  • Research Products

    (10 results)

All 2022 2021 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (4 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results)

  • [Int'l Joint Research] HDRCRP/Bogra Medical college(バングラデシュ)

    • Country Name
      BANGLADESH
    • Counterpart Institution
      HDRCRP/Bogra Medical college
  • [Journal Article] COVID-19 Community Survey, Knowledge, Attitude and Practice by Bangladeshi Population.2022

    • Author(s)
      Jesmin, S.; Maqbool, A.; Sohael, F.; Islam, M.M.; Matsuishi, Y.; Shima, T.; Shimojo, N.; Kawano, S.; Rahman, M.A.; Yamaguchi, N.; Moroi, M.
    • Journal Title

      Journal of Biomedical Research & Environmental Sciences

      Volume: 3(4) Pages: 367-372

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prevalence of Diabetes Mellitus Among Tuberculosis patients in Bangladesh.2022

    • Author(s)
      Mamun, M.A.; Maqbool, A.; Sohael, F.; Rahman, M.A.; Islam, M.M.;Shima, T.; Shimojo, N.; Kawano, S.; Yamaguchi, N.; Moroi, M.; Jesmin, S.
    • Journal Title

      Journal of Diabetes and its Complications

      Volume: 6(1) Pages: 1-8

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Covid-19 Vaccination status in Bogura district in Bangladesh.2022

    • Author(s)
      Jesmin, S.; Maqbool, A.; Sohael, F.; Islam, M.M.; Matsuishi, Y.; Shima, T.; Shimojo, N.; Kawano, S.; Rahman, M.A.; Yamaguchi, N.; Masao, M
    • Journal Title

      Journal of Biomedical Research & Environmental Sciences

      Volume: - Pages: -

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Covid-19, Co-morbidities and its impact on prognosis in a tertiary care hospital of Bangladesh.2022

    • Author(s)
      Mamun, M.A.; Maqbool, A.; Sohael, F.; Rahman, M.A.; Islam, M.M.; Shima, T.; Shimojo, N.; Kawano, S.; Yamaguchi, N.; Masao, M.; Jesmin, S
    • Journal Title

      Journal of Medical - Clinical Research & Reviews

      Volume: 6(2) Pages: 1-5

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prevalence of Metabolic Syndrome Components among EarlyAge onset Acute Coronary Syndrome Patients in Bangladesh.2021

    • Author(s)
      Jesmin, S.; Maqbool, A.; Sultana, S.N.; Sohael, F.; Islam, M.M.; Matsuishi, Y.; Shima, T.; Shimojo, N.; Kawano, S.; Rahman, M.A.; Yamaguchi, N.; and Moro, M
    • Journal Title

      Cardiology & Vascular Research

      Volume: 5(4) Pages: 1-8

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Role of angiogenesis in sepsis2022

    • Author(s)
      Jesmin S, Kawano S
    • Organizer
      nvited by Shuvo Diagnostic Center, Bogra, Bangladesh
    • Int'l Joint Research / Invited
  • [Presentation] Angiogenesis and sepsis: Therapeutic implication2021

    • Author(s)
      Jesmin S, Kawano S
    • Organizer
      A special online conference arranged by HDRCRP
    • Int'l Joint Research / Invited
  • [Presentation] Can sepsis complications be reversed?2021

    • Author(s)
      Jesmin S, Kawano S
    • Organizer
      Invited by TMSS, Bogra, Bangladesh
    • Int'l Joint Research / Invited
  • [Presentation] How do you deal with sepsis complications?2021

    • Author(s)
      Jesmin S, Kawano S
    • Organizer
      rranged by Sonar Desh Clinic, Bogra, Bangladesh
    • Int'l Joint Research / Invited

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Published: 2022-12-28  

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