Bio-permeable nerve conduits containing isogenic acellular muscle basal lamellae and bone marrow mesenchymal stem cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09493
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Kindai University
• Principal Investigator: KAKINOKI Ryosuke 近畿大学, 医学部, 教授 (20314198)
• Co-Investigator(Kenkyū-buntansha): 赤木 將男 近畿大学, 医学部, 教授 (00273441)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 末梢神経再生 / 人工神経 / 血行 / 細胞 / 足場 / 成長因子 / 同種無細胞性神経基底膜 / 自家無細胞性筋肉基底膜

Outline of Final Research Achievements

We have created an artificial nerve conduit model made of a silicone tube containing a sural vascular pedicle and decellularized allogenic nerve basal lamellae implanted by bone marrow derived mesenchymal stem cells. The nerve regeneration through the tube with a 20mm-interstump gap in a rat sciatic nerve was almost equal to that through a 20mm-long fresh nerve isograft. However, the use of allogenic nerves in the clinical setting is associated with several big problems that should be solved, including immunological responses, infectious disease transmission and ethical problems. The aim of this study was to investigate whether decellularized isogenic nerve basal lamellae (DIML) could be a substitute of decellularized allogenic nerve basal lamellae (DANL). Nerve regeneration occurred in the DANL and around the DIML. Scaffolds promoting nerve regeneration need the space that accommodates Schwann cell migration.

Free Research Field

手外科、末梢神経外科学

Academic Significance and Societal Importance of the Research Achievements

人工神経を作成するためには、神経再生に適した足場の開発が必須である。足場には、lamininなどの接着因子のみならず、シュワン細胞の移動を許容する空間が必要であることがわかった。本研究は、今後の人工神経開発の一助になると考える。