Human papillomavirus infection in urinary tract among the patients with bladder carinoma: Clarification of natural history and association of HPV infection and bladder carcinoma

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09520
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56030:Urology-related
• Research Institution: Kanazawa University
• Principal Investigator: Shigehara Kazuyoshi 金沢大学, 附属病院, 講師 (20595459)
• Co-Investigator(Kenkyū-buntansha): 川口 昌平 金沢大学, 附属病院, 助教 (30706883)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ヒトパピローマウイルス / 膀胱癌 / 性感染症

Outline of Final Research Achievements

The present study aimed to elucidate the appropriateness of spontaneously voided urine or bladder washing in screening HPV infection in the urinary bladder. Urine and bladder washing samples were obtained from 201 patients. After extracting DNA from both samples, HPV-DNA was examined using PCR. HPV genotyping was performed in the HPV-positive samples. HPV prevalence in urine and washing samples were 9.5% and 7.0%, respectively. HPV type distribution in both samples was not in agreement. The ISH analysis revealed that HPV-DNA signal was observed in urothelial cells of five (55.7%) of nine detectable HPV-positive cytological samples. Six (66.7%) of nine HPV-positive cases had HPV-DNA signals in tumor tissue. The use of washing samples was likely applicable for investigating HPV prevalence in the urinary bladder.

Free Research Field

性感染症

Academic Significance and Societal Importance of the Research Achievements

男性にとって尿路はHPV感染の好発部位の１つである。近年、膀胱癌とHPV感染との関連性についても議論されるようになったが、膀胱のHPV感染の状況は不明である。その理由として膀胱のHPV感染を調べるためには、麻酔科で膀胱粘膜の採取が必要であり、検体採取に侵襲性を伴うからと思われる。膀胱のHPV感染を調べるためには侵襲性の少ない検体採取法の開発が望まれる。本検討では、膀胱洗浄液が膀胱内HPV感染を反映していることを初めて立証した。膀胱洗浄液を用いることで、定期的に膀胱内HPV感染の状況を調査することが可能となり、膀胱HPV感染の自然史の解明に寄与することが期待できる。