Establishment of urinary biomarkers that can evaluate the pathological classification and severity of refractory bladder pain syndrome

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09571
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56030:Urology-related
• Research Institution: Tohoku University
• Principal Investigator: Kawamorita Naoki 東北大学, 大学病院, 講師 (00617524)
• Co-Investigator(Kenkyū-buntansha): 伊藤 明宏 東北大学, 医学系研究科, 教授 (70344661) ; 佐竹 洋平 東北大学, 大学病院, 助教 (70783984) ; 方山 博路 東北大学, 大学病院, 助教 (90466558)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 間質性膀胱炎 / 薬剤性膀胱炎 / 過活動膀胱 / 膀胱虚血

Outline of Final Research Achievements

Interstitial cystitis, drug-induced cystitis, and overactive bladder are sometimes difficult to differentiate, and urinary biomarkers (BM) are awaited. Using the bladder ischemia model (AI model), we explored BM in bladder tissue. In the AI model, multiple mRNAs such as sGC, CCL7.24, and epha7 were elevated in the bladder mucosa. After creating the AI model, by changing the diet to a normal diet, an improvement in bladder blood flow and a tendency to prolong the micturition interval were observed. In addition, an increase in CD31 mRNA was observed. On the other hand, no significant difference was detected in urine. From the above, we found a candidate pathology-related BM in the bladder mucosa, However we did not reach the point of identification in the urine. We believe that a different approach is required for differentiation based on urinary metabolites.

Free Research Field

排尿障害

Academic Significance and Societal Importance of the Research Achievements

間質性膀胱炎は、薬剤性膀胱炎や過活動膀胱（OAB）との鑑別が困難である。尿中バイオマーカー（BM）が待望されている。我々はOABを呈する膀胱虚血ラットモデル（AIモデル）の膀胱粘膜でsGC、CCL7,24、epha7などのmRNAの上昇を認めた。AIモデルを作成後、食餌を通常食に変更すると膀胱結血流の改善と、過活動の改善が認められた。また、CD31のmRNAの上昇を認めた。しかしながら、尿中での有意差は検出されなかった。以上より、疾患差別化のBIの候補を見出したが、尿中BMの同定までにはいたらなかった。一方、膀胱虚血と高脂肪職食が引き起こすOABは、食事の改善により改善する可能性を示した。