2022 Fiscal Year Final Research Report
Therapeutic strategy of advanced ovarian cancer by targeting carbonyl reductase 1
| Project/Area Number |
20K09589
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56040:Obstetrics and gynecology-related
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | Carbonyl reductase 1 / 卵巣癌 / エクソソーム / トランスジェニックマウス / プロテオーム解析 / パスウェイ解析 / eIF2シグナル / 質量分析解析 |
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| Outline of Final Research Achievements |
We created transgenic mice overexpressing mouse Cbr1 (mCbr1), and investigated the expression of mCbr1 in two strains of these mice. One strain showed overexpression of mCbr1 in various tissues, while the other strain exhibited overexpression specifically in the heart. Mass spectrometry analysis using the heart tissue identified 73 proteins that correlated with overexpression of mCbr1, including voltage-dependent anion channels. We believe that the mCbr1-Tg mouse will be useful for analyzing the physiological significance of CBR1 in various organs. Overexpression of CBR1 in ovarian cancer cells was found to significantly affect eIF2 signaling. This suggests that CBR1 may be involved in tumor suppression through multiple pathways.
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| Free Research Field |
産科婦人科学
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| Academic Significance and Societal Importance of the Research Achievements |
Carbonyl reductase 1 (CBR1) の生理的意義を解明する一助として、マウスCbr1 (mCbr1) を過剰発現するトランスジェニックマウス (mCbr1-Tgマウス) の作製に成功した。mCbr1-Tgマウスは、各臓器におけるCBR1の生理的意義を解析するのに有用であると考える。CBR1が腫瘍抑制に複数の経路を介して関与している可能性が示唆され、新たな治療戦略の可能性を見出した。
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