The study of human trophoblast differentiation: epigenetic transcription regulation of aromatase gene

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09632
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56040:Obstetrics and gynecology-related
• Research Institution: Fujita Health University
• Principal Investigator: Ishihara Naoe (琴村直恵) 藤田医科大学, 医学部, 研究員 (50571791)
• Co-Investigator(Kenkyū-buntansha): 石原 悟 藤田医科大学, 医学部, 講師 (00300723) ; 下野 洋平 藤田医科大学, 医学部, 教授 (90594630)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 胎盤 / 栄養膜細胞 / 分化 / アロマターゼ / 遺伝子発現

Outline of Final Research Achievements

During human placental trophoblast differentiation, the aromatase gene expression is significantly upregulated. To study the transcription mechanism of the aromatase gene in trophoblast cells, we used undifferentiated trophoblast model cell line (JEG-3). We found that a universal methyltransferase inhibitor treatment induces aromatase gene expression and changes chromatin structure in JEG-3 cells.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

胎盤栄養膜細胞のモデル細胞を用いて、アロマターゼ（エストロゲン合成酵素）の遺伝子発現がエピジェネティックに制御される機構の一端を明らかにした。