Reconstruction of vocal cord layer structure by cell stacking using gelatin hydrogel particles

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09755
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56050:Otorhinolaryngology-related
• Research Institution: Clinical Research Center Kurashiki Central Hospital
• Principal Investigator: Yamada Koichiro 公益財団法人大原記念倉敷中央医療機構(臨床医学研究所 臨床医学研究開発部), クリニカルサイエンスリサーチグループ, 研究員 (60815257)
• Co-Investigator(Kenkyū-buntansha): 岸本 曜 京都大学, 医学研究科, 助教 (80700517)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 声帯 / ゼラチンハイドロゲル / 細胞シート / 線維化

Outline of Final Research Achievements

In this study, cell sheets were prepared using cells isolated from canine vocal folds, and layered cell sheets were prepared by stacking them using gelatin hydrogel particles. Layered cell sheet was successfully transplanted to the injured vocal fold. Although historlogical change was not identified with cell sheet transplantation, it resulted in reduced atrophic change and preserved mucosal vibration. It was suggested that layered cell sheets made from gelatin hydrogel particles may be effective for regeneration of the vocal fold mucosa.

Free Research Field

耳鼻咽喉科学

Academic Significance and Societal Importance of the Research Achievements

本研究では声帯粘膜より採取した細胞とゼラチンハイドロゲル粒子を用いることで積層細胞シートを作製した。この積層細胞シートを移植することによる声帯粘膜再生効果が示されれば、難治性の声帯の線維性疾患の新たな治療法開発につながる可能性がある。また、その技術は他臓器における線維化疾患に対しても応用できる可能性がある。