2022 Fiscal Year Final Research Report
Mechanisms of inflammatory VEGF production via IL-17A in diabetic retinopathy
Project/Area Number |
20K09840
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 56060:Ophthalmology-related
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Research Institution | National Defense Medical College |
Principal Investigator |
Takeuchi Masaru 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究施設、病院並びに防衛, 眼科学, 教授 (40260939)
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Co-Investigator(Kenkyū-buntansha) |
伊藤 正孝 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究施設、病院並びに防衛, 再生発生学, 准教授 (30534896)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 糖尿病網膜症 / オステオポンチン / 網膜血管透過性 / ZO-1 / Claudin-5 |
Outline of Final Research Achievements |
Serum and retinal OPN concentrations in streptozotocin-induced diabetic mice (STZ mice) were significantly higher than those in control mice, and increased vascular hyperpermeability in the retina of STZ mice was reduced by intravitreal injection of anti-OPN neutralizing antibodies (anti-OPN Abs). The expression of claudin-5 and ZO-1, which consist of the retinal vascular barrier, was attenuated in the retina of STZ mice and ameliorated by anti-OPN Ab IVI. On the other hand, OPN Ab IVI inhibited neither gene expression nor protein level of vascular endothelial growth factor (VEGF) in the eye, indicating that retinal vascular permeability enhancement by OPN is VEGF-independent.
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Free Research Field |
糖尿病網膜症
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Academic Significance and Societal Importance of the Research Achievements |
Our results suggest that OPN induces tight junction disruption and vascular hyperpermeability under diabetic conditions. Targeting OPN may be an effective approach to manage diabetic retinopathy.
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