Application of regenerative therapy to skin ulcer caused by deep dermal burn for scarless healing

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K09862
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Okano Junko 滋賀医科大学, 医学部, 講師 (50447968)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 創傷治癒 / 骨髄細胞 / 組織再生 / スキャフォールド

Outline of Final Research Achievements

In the present study, we determined that the application of GS in deep injury made vasculogenesis possible by bone marrow-derived cells (BMDCs), leading to regeneration of the fascia as well as the skin with appendages and less contracture deformities compared with control wounds. Furthermore, the Y-chromosome analyses were utilized to clarify the contribution of BMDCs in the homeostasis of the epidermis in the skin. Consequently, it is worthwhile to note BMDC-derived keratinocytes are significantly detected in GS (+) rats, which were sporadically present in GS (-) rats. It appears that the microenvironments provided by GS are suitable for BMDCs transdifferentiation into keratinocytes.

Free Research Field

形成外科学

Academic Significance and Societal Importance of the Research Achievements

多くの全身熱傷の場合、分層植皮が利用されるが、治癒しても皮膚が薄いため
拘縮をきたし、さらに皮膚付属器は再生されない。この問題を解決するため、本研究では創に組織再生をもたらすスキャフォールドの開発を目指し、動物モデルにて皮膚付属器を含めた皮膚、軟部組織の再生に成功した。次はヒトへの外挿を目指す。