2021 Fiscal Year Research-status Report
APPLICATION OF RvD2 AS A REGENERATIVE DIRECT PULP CAPPING MATERIAL
| Project/Area Number |
20K09938
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大森 一弘 岡山大学, 大学病院, 講師 (20549860)
山城 圭介 岡山大学, 大学病院, 講師 (30581087) [Withdrawn]
高柴 正悟 岡山大学, 医歯薬学域, 教授 (50226768)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | Vital pulp therapy / Resolving D2 / Reparative dentin / Dental pulp cells |
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| Outline of Annual Research Achievements |
Vital pulp therapy (VPT)’s aim is reparative dentin production after pulp injury. Current VPT agents have biocompatibility problems. Previously we proved that RvD2, an anti-inflammatory lipid mediator, produced in vivo from polyunsaturated fatty acids healed apical periodontitis (AP). Having analgesic, angiogenic, bacterial clearance effects, RvD2 is the "ideal VPT agent". In 2020, we reported reparative dentin (RD) after RvD2 and Ca(OH)2 application (rat model). IMH results showed GPR18’s expression in RvD2 group. This year we added this agent in dental pulp cells, finding decreased expression of Tnf-α and Il-1β, suggesting that RvD2 had an anti-inflammatory effect. RvD2 may establish a favorable environment for the formation of RD in the dental pulp by its anti-inflammatory effects.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
The following goals have been achieved. In vivo experiments, 1) the pulpotomy rat model has been standardized, 2) the dentin bridge regeneration at the pulp injury after RvD2 application as a VPT agent has been found, 3) immunohistochemistry confirmed the presence of GPR18 on RvD2 groups.
In vitro, RvD2 decreased the gene expression level of Tnf-α and Il-1β in dental pulp cells, which results suggested anti-inflammatory effect of RvD2 in dental pulp cells.
Because of COVID-19 pandemic, it was not possible to check all these factors, however some of the obtained results until now were reported at the International Association of Dental Research (IADR) in July 2021.
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| Strategy for Future Research Activity |
In vitro, to examine the effects of RvD2 on dental pulp cells we will perform using real-time PCR and ELISA the expression of the following markers: anti-inflammatory cytokines (IFN-γ, IL-10, IL-17, TGF-β), inflammatory cytokines (IL-1β, IL-6, IL-8, TNF-α), angiogenesis and tissue regeneration cytokine (VEGF), hard tissue formation (wnt3a, wnt10a, β-catenin), analgesic effect (TRPA1, substance P), dentin related markers (DSPP, DMP-1, alkaline phosphatase, osteocalcin), RvD2 receptor (GPR18). Furthermore, in vivo experiments, we will observe the expression of these marker using immunohistochemistry. However, the results obtained until this year show that the effects of this materials are so amazing to protect the viability of dental pulp. Thus, a manuscript is underway to submit to an international journal, and it is expected to obtain comments and suggestion to improve this study.
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| Causes of Carryover |
Because of COVID-19 pandemic,it was not possible to conduct the the planned in vivo experiments so we will use the remained money for the experiments next Fiscal Year.
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[Presentation] Resolvin D2 Promotes the Formation of Calcified Tissue on Pulp2021
Author(s)
Mitsuhiro Yoneda, Zulema Rosalia Arias Martinez, Shin Nakamura, Kentaro Okamoto, Masahiro Ito, Kazuya Tamura, Hidetaka Ideguchi, Kazuhiro Omori, Keisuke Yamashiro, Tadashi Yamamoto, Shogo Takashiba.
Organizer
2021 IADR/ AADR/ CADR General Session
Int'l Joint Research
