Development of control method for root caries focusing on the functions of oral biofilm microbiota

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K09953
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Osaka University
• Principal Investigator: Asahi Yoko 大阪大学, 大学院歯学研究科, 助教 (50456943)
• Co-Investigator(Kenkyū-buntansha): 三浦 治郎 大阪大学, 歯学部附属病院, 助教 (70437383)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: バイオフィルム / 根面う蝕 / 細菌叢 / 機能

Outline of Final Research Achievements

Biofilms were collected from root caries lesions, and the function of biofilm bacteria associated with root caries was investigated. The results suggested that microbiota associated with root caries lesion may have a different function from the biofilm formed on the healthy exposed root surface.
On the other hand, as a result of investigating the effects of zinc-glass-containing glass ionomer cement on biofilms, it was shown that the number of viable bacteria and the thickness of the biofilm of Actinomyces naeslundii decreased compared to the control group, and that gene expression changed.

Free Research Field

保存治療系歯学

Academic Significance and Societal Importance of the Research Achievements

高齢者の保有歯数が増加し，加齢や歯周病による歯肉退縮に伴う根面う蝕の急増が歯科において深刻な問題となっており，根面う蝕に対する効果的な予防・制御法が切望されている。本研究により，根面う蝕病変部によりバイオフィルム細菌叢の機能が変化する可能性が示された。得られた結果は，根面う蝕の病態解明や新たな抑制法の開発の一助となりうる。