Induction of odontoblastic differentiation of stem cells of the apical papilla by S1P and application to regenerative endodontic procedures

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K09969
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Fukuoka Dental College
• Principal Investigator: Matsuzaki Etsuko 福岡歯科大学, 口腔歯学部, 教授 (20432924)
• Co-Investigator(Kenkyū-buntansha): 高橋 富美 産業医科大学, 医学部, 教授 (50274436) ; 阿南 壽 福岡歯科大学, 口腔歯学部, 病院顧問 (80158732)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: sphingosine-1-phosphate / SCAP / differentiation / odontoblast

Outline of Final Research Achievements

During odontogenic differentiation of mouse stem cells of the apical papilla (iSCAP), we found that S1P and BMP-9 induced mRNA and secreted protein expression of differentiation markers including DSPP, DMP1, and MEPE, and promoted mineralization through S1P/S1PR1 signaling pathway. In rat immature teeth, many S1PR1-positive cells were observed in the pulp tissue and the adjacent odontoblast-like cell area, as well as in the apical papilla. Furthermore, in the regenerative endodontic procedures model, an increase in root canal wall thickness and the formation of bone-like hard tissue near the root apex were observed due to the S1PR1 agonist.
S1P/S1PR1 pathway may be involved in promoting odontogenic differentiation of SCAP and root formation after regenerative endodontic procedures.

Free Research Field

歯内療法学、歯科保存学

Academic Significance and Societal Importance of the Research Achievements

本研究で見出したS1P/S1PRシグナルによる新たなSCAPの象牙芽細胞分化促進作用は、再生歯内療法後の歯根形成におけSCAPの賦活化と象牙質-歯髄複合体の再生に応用できる可能性がある。