Clarification of virulence factor involved in the chronicity of osteomyelitis of the jaw

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K10112
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Michi Yasuyuki 東京医科歯科大学, 大学院医歯学総合研究科, 准教授 (70376755)
• Co-Investigator(Kenkyū-buntansha): 中川 一路 京都大学, 医学研究科, 教授 (70294113) ; 渡辺 孝康 日本大学, 歯学部, 講師 (70725514)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 複合感染症 / 顎骨骨髄炎 / メタ16S解析 / メタトランスクリプトーム解析

Outline of Final Research Achievements

Chronic osteomyelitis of the jaw (COMJ) is an inflammation of the jaw bone that progresses from sclerosis to frustration. We previously classified sequestrum into three stages, with no exposure designated as stage II. In this study, we examined the RNA:DNA ratio of bacteria in COMJ to identify active bacteria. Eight phyla were predominant in each stage, but their composition varied from sample to sample. Diverse compositions were also observed at the genus level. However, the RNA:DNA ratios of Actinomyces and Fusobacterium spp. were extremely high in Stage II, but almost zero in Stages I and III.

Free Research Field

口腔感染症

Academic Significance and Societal Importance of the Research Achievements

本研究では、難治性を示す顎骨骨髄炎において、病気の発症や進行に関連し得る細菌種を同定した。さらに、16S rDNA, rRNAを用いた模擬的なメタトランスクリプトームを行うことで病変に存在する細菌種の活動性を評価する解析プロトコルを利用した。本プロトコルは、細菌腫の保有する病原因子の同定はできないものの、メタトランスクリプトームと比較して簡便に細菌種活動性を評価できる利点を有しており、口腔内おける他の複合感染症にも応用できると考える。