2022 Fiscal Year Final Research Report
Improvement method of bone quality using stem cell culture conditioned medium-derived factors that control stem cell/macrophage dynamics
Project/Area Number |
20K10113
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 57060:Surgical dentistry-related
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Research Institution | Gifu University (2022) Niigata University (2020-2021) |
Principal Investigator |
Katagiri Wataru 岐阜大学, 大学院医学系研究科, 准教授 (10437030)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | マクロファージ / 骨再生 / 培養液 / 幹細胞 |
Outline of Final Research Achievements |
depMSC-CM was prepared by removing MCP-1 from MSC-CM. Macrophages (BMM) were collected and cultured from rat femurs. Polarity was confirmed by culturing BMM in the presence of MSC-CM and depMSC-CM. Human MSCs were cultured in the presence of each CM, and osteogenesis-related gene expression was examined. In a rat calvaria bone defect model, each CM was implanted and bone formation was examined. MSC-CM contains MCP-1, and the expression of anti-inflammatory macrophage markers in BMM cultured in the presence of MSC-CM and the expression of osteogenesis-related genes in human MSCs were enhanced. In the transplantation experiment, bone formation was promoted in the MSC-CM group, and immunohistochemical evaluation showed an increase in the number of anti-inflammatory macrophages.
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Free Research Field |
口腔外科
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Academic Significance and Societal Importance of the Research Achievements |
MSC-CMに含有されるMCP-1がマクロファージ極性転換およびそれに続く骨形成に重要な役割を担っていることが示唆された。 MSC-CMによる骨形成に関わる因子の同定は組織再生のメカニズムを明らかにするばかりでなく、創薬の基盤研究ともなり得る。幹細胞ニッチにおける造血幹細胞-間葉系幹細胞間の相互作用などを含め新たな骨再生の戦略として今後も研究を継続する予定である。
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