Identification and molecular analysis of calcineurin binding protein related to osteo immunity by inflammation

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K10121
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Kyushu University
• Principal Investigator: Sugiyama Goro 九州大学, 歯学研究院, 助教 (00722828)
• Co-Investigator(Kenkyū-buntansha): 藤原 稔史 九州大学, 大学病院, 助教 (20644800) ; 山田 朋弘 九州大学, 歯学研究院, 准教授 (60335619)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 骨免疫 / 骨代謝 / 骨芽細胞

Outline of Final Research Achievements

Calcineurin is major protein phosphatase, which play important role for system of osteo immunity. In osteoblasts, we have experiments of westernblotting analysis, which is investigation for NFAT activity. Dephosphorylation of NFAT is induced, and suggested activation of NFAT-Calcineurin signals. We investigated calcineurin binding protein, and find NMT1, which is important molecule for immuno response. NMT1 is a transferase of protein myristoylation, and calcineurin is also catalyzed by the enzyme because of calcineurin have specific amino-seqence for the reaction. These results suggested that molecular change in calcineurin and in regulatory mechanisms for immunity of osteoblasts.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

顎骨骨髄炎などの骨炎症性疾患は難治性であり、その病態や分子生物学的機序はほとんど明らかになっていない。骨表面に存在する骨芽細胞が骨免疫の主体を担うと考えられ、この細胞内分子挙動の解明に繋がれば、病態把握や治療薬へのアプローチとして学術的、社会的意義が大きい。また、予防的に対応する手法や阻害剤などを用いたアプローチへ発展する可能性がある。