Effect of extracellular matrix protein of epithelial rests of Malassez supernatants

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K10213
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: SAITOH Masato 北海道医療大学, 歯学部, 教授 (50337036)
• Co-Investigator(Kenkyū-buntansha): 谷村 明彦 北海道医療大学, 歯学部, 教授 (70217149) ; 安彦 善裕 北海道医療大学, 歯学部, 教授 (90260819)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: マラッセ上皮遺残細胞 / アメロジェニン

Outline of Final Research Achievements

The periodontal tissue supporting the teeth is composed of gingiva, alveolar bone, cementum, and periodontal ligament. The periodontal ligament contains mesenchymal cells such as fibroblasts, osteoblasts, and cementoblasts, as well as epithelial rest of Malassez (ERM) cells, which are derived from the odontogenic epithelium of the ectoderm, and are unique in that they remain present throughout root formation. ERMs are derived from the odontogenic epithelium and express the enamel matrix proteins amelogenin and ameloblastin, which are involved in enamel formation. In this study that the addition of culture supernatant of Malasse epithelial remnant cells to mouse tooth embryos induces enamel hydroxyapatite.

Free Research Field

小児歯科

Academic Significance and Societal Importance of the Research Achievements

これまでに、エナメル質の形成に関わっているタンパク質は明らかになっているものの、生体内での形成のため不明な点が多かった。今回我々の研究で、培養歯胚にエナメル質形成タンパクの一つであるアメロジェニンを添加することにより、エナメル質ハイドロキシアパタイトの形成を誘導することを明らかにした。