Elucidatin of the mechanism of oligodontia casued by Wnt mutation using in vivo assay

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K10227
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: The University of Tokushima
• Principal Investigator: YASUE Akihiro 徳島大学, 大学院医歯薬学研究部(歯学域), 徳島大学専門研究員 (80380046)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 歯の発生 / ゲノム編集 / 歯牙欠損症 / Wnt10a

Outline of Final Research Achievements

Although Wnt10A and Wnt10B have been reported as causative genes of human dentition, knockout (KO) mice do not cause tooth loss. When created, unlike the original deletion mutant, the maxillary incisors and third molars were deleted. The maxillary second molars and mandibular incisors were also dwarfed, and the crown morphology was flat and taurodont-like in Wnt10a-/-. This result also suggests functional overlap/complementation between Wnt10a and Wnt10b paralogues.

Free Research Field

歯の発生

Academic Significance and Societal Importance of the Research Achievements

Wnt10aとWnt10bそれぞれのシングルノックKOマウスでは歯数減少を示さなかったが、ダブル変異体では歯数の様々なバリエーションが示された。ダブルKOマウスを作製したところ、単独欠失変異体と異なり、上顎切歯・第三臼歯が欠失した。上顎第二臼歯・下顎切歯も矮小化しており、また、歯冠形態はWnt10a-/-同様平坦でタウロドントを呈し、さらに各々の変異体では認められなかった体長の減少も生じた。この結果は、Wnt10aとWnt10bのパラログ間での機能の重複・補完を示唆するものでもある。