2022 Fiscal Year Final Research Report
Analysis of epithelial-mesenchymal interactions during tooth development by proteomic approch
Project/Area Number |
20K10237
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 57070:Developmental dentistry-related
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Research Institution | The Nippon Dental University |
Principal Investigator |
Shimomura Junko 日本歯科大学, 新潟生命歯学部, 教授 (00386286)
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Co-Investigator(Kenkyū-buntansha) |
森田 貴雄 日本歯科大学, 新潟生命歯学部, 教授 (20326549)
大島 勇人 新潟大学, 医歯学系, 教授 (70251824)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | プロテオミクス / 歯の発生 / マウス |
Outline of Final Research Achievements |
Using a proteomic approach, this study comprehensively analyzed protein expression in epithelial and mesenchymal tissues of the tooth germ during development. First molar tooth germs from embryonic day 14 and 16 ICR mouse embryos were collected and separated into epithelial and mesenchymal tissues by laser microdissection. Mass spectrometry of the resulting proteins was carried out and three types of highly expressed proteins [ATP5B, RACK1, and CALR] were selected for immunohistochemical analysis. The expression profiles of these proteins were subsequently evaluated during all stages of amelogenesis using the continuously growing incisors of 3-week-old male ICR mice. Interestingly, these three proteins were specifically expressed depending on the stage of amelogenesis.
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Free Research Field |
小児歯科学
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Academic Significance and Societal Importance of the Research Achievements |
本研究により、3種類のタンパク質のエナメル質形成過程における局在の違いを確認できたことから、今後さらにより詳細な機能解析へと繋げられる点に大きな学術的意義がある。 また、本研究の成果は種々の因子に起因する歯の先天欠如や過剰歯、エナメル質形成不全症を含む形態異常歯など、小児歯科臨床上問題となっている歯の発生・発育異常の発症メカニズムの解明に繋がることから、将来歯に障害がある小児の治療に大きく貢献でき、社会的意義があると考える。
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