Establishment of a novel trauma therapy using dental pulp stem cells

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K10239
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Aichi Gakuin University
• Principal Investigator: Ishizaka Ryo 愛知学院大学, 歯学部, 歯学部研究員 (00705197)
• Co-Investigator(Kenkyū-buntansha): 林 勇輝 愛知学院大学, 歯学部, 講師 (10756547) ; 庵原 耕一郎 国立研究開発法人国立長寿医療研究センター, 研究所 幹細胞再生医療研究部, 室長 (60435865)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 再生歯科治療 / 歯の外傷 / 過重力

Outline of Final Research Achievements

Trauma is frequently encountered in the field of pediatric dentistry. Because of the wide range of disabilities caused by trauma, there is a wide spectrum of symptoms. Therefore, regenerative dentistry is required to restore function. It is known that hypergravitational cell culture accelerates differentiation. Therefore, we decided to compare the expression kinetics of Wnt10a, vascular and dentin markers during vascular induction of dental pulp stem cells. wnt10a increased transiently during early differentiation up to 72 hours in the dental pulp, but was expressed with temporal specificity from day 3 to 21. Its kinetics were consistent with vessels in the early period and dentin in the long term. The gene-level expression of Wnt10a was also accelerated by hypergravity.

Free Research Field

小児歯科学

Academic Significance and Societal Importance of the Research Achievements

再生医療を適応するための問題点として、細胞の培養期間や費用の問題が挙げられる、本研究の結果から歯髄幹細胞が血管および象牙質へと分化成熟する際にwnt10aが関与していることが示唆された。またその発現は過重力により早まることが示唆された。本研究が完成すれば、分化した細胞を迅速かつ安定して供給できるようになる可能性がある。従って、細胞加工に対するコストを削減可能となる。より多くの症例に再生医療が適用可能になると考えられる。