2022 Fiscal Year Final Research Report
Development of a Photodynamic Diagnosis Method for Oral Squamous Cell Carcinoma Using 5-ALA
| Project/Area Number |
20K10256
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57080:Social dentistry-related
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 口腔がん / 光線力学的診断 / 擦過細胞診 |
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| Outline of Final Research Achievements |
A fluorescent plate reader was used to measure the fluorescence intensity of a human oral cancer-derived squamous cell carcinoma cell line and a human normal oral mucosa cell line (NHOK) over time. Fluorescence intensity was significantly stronger at In addition, addition of deferoxamine mesylate (DFO) was tried to obtain strong fluorescence intensity with a smaller amount of cells. As a result, a significant difference could be obtained with a smaller amount of cells. The results obtained from this study have realized the possibility of reliably performing oral cancer diagnosis in a chairside setting of a dental clinic without administering 5-ALA to the living body and completely eliminating biohazards.
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| Free Research Field |
歯科臨床検査医学
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| Academic Significance and Societal Importance of the Research Achievements |
口腔がんの早期発見には医療者間の診断能力格差が発生することが少ない診断方法を構築することは極めて重要である。擦過細胞診は病変が直視直達できるという口腔領域の特性や患者側への侵襲が低いということから有用なツールである。本研究は口腔がんに対して擦過細胞診と光線力学的診断（以下PDD）を組合せ新たな診断方法を確立することを目的とした。
本研究から得られた結果により、歯科診療所のチェアサイドにおいて5-ALAを生体へ投与することなく生体為害性を完全に排除した状況で口腔癌診断を確実に実施できる可能性が現実化した。
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