Development of high-throughput analytical method for urinary aromatic amines for dermal exposure assessment

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K10451
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 58020:Hygiene and public health-related: including laboratory approach
• Research Institution: Hiroshima University
• Principal Investigator: Nagao Masataka 広島大学, 医系科学研究科(医), 教授 (80227991)
• Co-Investigator(Kenkyū-buntansha): 奈女良 昭 広島大学, 医系科学研究科(医), 教授 (30284186)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 労働衛生 / 分析化学 / リスクアセスメント / 芳香族アミン

Outline of Final Research Achievements

In this study, aniline, toluidine (o-, m-, p-), anisidine (o-, m-, p-), naphthylamine, and 4,4'-methylenebis(2-chloroaniline) (MBOCA) were used as model compounds for aromatic amines, and amino phenols (o-, m-, p-) as metabolites. Aromatic amines in urine were trifluoroacetylated in the extraction solution after alkaline extraction, while metabolites in urine were directly acylated with acid anhydride and alkaline solution to simultaneously derivatize amino and hydroxyl groups before extraction with an organic solvent. With this method, it was possible to analyze aromatic amines and their metabolites easily without evaporation and drying. In the future, it is expected that this method will also be used for the analysis of similar compounds.

Free Research Field

法医学

Academic Significance and Societal Importance of the Research Achievements

本成果では、健康被害をもたらす恐れのある芳香族アミンの代表であるアニリンの簡便なるガスクロマトグラフ/質量分析法が構築できた。有害性の指摘されている芳香族アミンは多岐にわたり、適正なる分析機器あるいは手法を選択した網羅的な芳香族アミン類のスクリーニング法の開発は喫緊の課題であると指摘されている。本研究成果は、有害な芳香族アミンや代謝物のスクリーニング分析にも応用可能であり、健康被害を未然に防止できるような検査法の整備を加速させ、健康被害を引き起こす化学物質を網羅的に分析できる方法の構築・整備に貢献できると期待される。